Page 533 - Book Hosokawa Nanoparticle Technology Handbook

21 DEVELOPMENT OF FUNCTIONAL SKINCARE COSMETICS APPLICATIONS

0.05mm

(a) Non-administration of Theophyllin (b) Theophyllin only (c) NanoCrysphere + Theophyllin
(before pseudo-sunburn) (3 days later) (3 days later)
(After pseudo-sunburn
by UV radiation)
(Evaluation: Prefectural University of Hiroshima/Miwa lab.)

Figure 21.5
SEM photos (1000 ) of melanocyte derived from human cells (HMV-II) with and without Teophylin and nanospheres
treatment.

1mm

(a) Before UV-A irradiation (b) After UV-A irradiation: (c) After UV-A irradiation
(control) wrinkle level +++ (administration of
Nanocrysphere TM):
wrinkle level +-

(Evaluation: Prefectural University of Hiroshima/Miwa lab.)

Figure 21.6
Antiaging effect of NanoCrysphere™ on human skin biopsies before and after UV-A irradiation. UV-A: 3 J/cm ,
2
2 times/min, 2 times/day. Concentration of NanoCrysphere: 200 l. Human skin biopsies: ear skin from 55-year-old woman.

diffusion chamber, and then exposed to the UV-A irra- penetrated through the skin and helped to form vita-
2
diation at 3 J/cm twice a day for five consecutive days. min derivatives in the dermis, which eliminated the
After receiving the UV-A irradiation for some time, wrinkle causing UV-A-derived active oxygen.
large wrinkles shown as deep shadows on the skin PLGA nanospheres can encapsulate low skin per-
(Fig. 21.6a) and small wrinkles shown as narrow but meability drugs and deliver them to deep skin (der-
deep-cut indentation (Fig. 21.6b) could be observed. mis). We applied this basic technology to skincare
The wrinkle-line histograms were also irregular and products and demonstrated their skin whitening and
rough, as furrows and hills. However, if the functional anti-aging efficacy. The PLGA nanosphere can be a
beauty essence (0.1% NanoCrysphere in saline, 200 l) useful transdermal drug delivery carrier.
was applied on the skin sample before UV-A irradia-
tion, there were hardly any large or small wrinkles
formed on the skin sample (Fig. 21.6c), and the wrinkle- References
line histogram was comparatively regular with fine
grooves (texture), indicating that the wrinkle forma- [1] Yomiuri Shinbun Tyokan, pp. 2, 11 January, 2005 Oyobi
tion was remarkably suppressed. This was likely Yomiuri Shinbun Yukan, pp. 3, 1 March, “Kyokushou
because the encapsulated nanospheres significantly kapuseru shiwa tori keshouhin”.

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