Page 375 - Organic Electronics in Sensors and Biotechnology

352 Chapter Nine

CWD Sheep scrapie
(a)
Separated LCP chains
1.9

1.7
Ratio 532/639 1.5 CWD

1.3
1.1 Sheep scrapie
0.9
Aggregated LCP chains 0.6 0.7 0.8 0.9 1.0
Ratio 532/Emax Nonplanar
Planar LCP backbone
LCP backbone
(b)

150 PTAA
Native PrP
Fluorescence (RFU) 100
PrP fibrils A
PrP fibrils B

50

0
500 550 600 650 700
Wavelength (nm)
(c)
FIGURE 9.10 (a) Fluorescent images of prion deposits associated with
distinct prion strains, chronic wasting disease (CWD, left) and sheep scrapie
(right), which have been stained by PTAA. (b) Correlation diagram of the
ratios, R532/639 and R532/E , of the intensity of the emitted light from
max
PTAA bound to prion deposits originating from individual mice infected with
CWD (four generations denoted with black symbols, Q, x, V, x) or sheep
scrapie (two generations denoted with purple symbols, squares and
diamonds). (c) Emission spectra of PTAA in buffer (black), PTAA bound to
native (blue) or two different forms of fi brillar recombinant mouse PrP (green
and purple). [Part (c) reproduced with permission from Ref. 112. Copyright
2007, Nature Publishing Group.] (See also color insert.)