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temperature changes in the diode and also to variations in integration time [6]. The noise from
integrated dark current can therefore be reduced by cooling the diode array. Thus, by digital background
subtraction and the use of a Peltier cooling system to reduce the temperature of the diodes, the use of
the diode array for fluorescence spectroscopy becomes possible.
Figure 7.21
Chromatograms of a Seven-component
Mixture of Polycyclic Aromatic Hydrocarbons
Reprinted with permission from J. Wegrzyn, G. Patonay, M. Ford and I. Warner,
Anal. Chem., 62(17)(1990)1754, Copyright 1990 American Chemical Society
Diode arrays were first examined for use in liquid chromatography detection in the mid-1970s.
Wegrzyn et al. [61 were one of the earlier workers to explore the possibilities of a diode array for
fluorescence measurements in liquid chromatography and a diagram of their tandem
