Page 325 - Thermodynamics of Biochemical Reactions

Chapter 6 Systems of Biochemical Reactions

6.1 Make the stoichiometric number matrix for reactions 6.5-17 to 6.5-19 and use it to print out these reactions.

6.2 Calculate the pathway from glucose to carbon dioxide and water in terms of (1) the net reaction for glycolysis, (2) the net
reaction catalyzed by the pyruvate dehydrogenase complex, (3) the net reaction for the citric acid cycle, and (4) the net
reaction for oxidative phosphorylation.

6.3 (a) Type in the stoichiometric number matrix for glycolysis at a specified pH that is given in Fig. 6.1. (b) Use nameMa-
trix to type out the 10 reactions. (c) Use equation 6.1-3 to calculate the net reaction for glycolysis. (d) Calculate a basis for
the conservation matrix for glycolysis and row reduce it to determine the number of components.

6.4 The following reaction is carried out enzymatically at 298.15 K, pH 7, and 0.25 M ionic strength.
ATP + glucose = glucose6phos + ADP
The initial concentrations of ATP and glucose are 0.001 M. What is the equilibrium composition? Solve this problem using
Mathernatica in two ways.

6.5 Calculate the apparent equilibrium constant for the net reaction for the first five reactions of glycolyis that is, reactions
6.6-1 to 6.6-5) using equation 6.1-6. Check this by simply using the net reaction.

6.6 A liter of aqueous solution contains 0.01 mol Hz PO4 -, 0.01 mol HP04 '-, and 0.01 mol Mg2+. Assuming that the ionic
strength is zero, what is the equilibrium composition of the solution in terms of species at 298.15 K?

6.7 When 0.01 M glucose 6-phosphate is hydrolyzed to glucose and phosphate at 298.15 K in the pH range 9-10, only four
species have to be considered.
GlcP2- + Hz 0 = Glc + HP04 2-
What is the equilibrium composition assuming the ionic strength is zero?

6.8 A liter of aqueous solution contains 0.02 mol phosphate and acid and NaCl are added to bring it to pH 7, and 0.25 M
ionic strength. What is the equilibrium composition in terms of phosphate species?

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