Page 43 - Vibrational Spectroscopic Imaging for Biomedical Applications
P. 43
20 Cha pte r O n e
Molecular
-1
Feature Position (cm ) Assignment 33 Origin
−1
Peak ratio 1238:1542 1238 cm : CN DNA/RNA
stretching, CH wagging, Protein
2
assymetric PO −
2
–1
stretching, 1542 cm :
NH bending
−1
Peak area 1426–1482 1456 cm : assymetric Protein
CH bending
3
−1
Center of 1300–1358 1338 cm : CH Protein
2
gravity wagging (Amide III)
−1
Peak ratio 1080:1234 1080 cm : symmetric DNA/RNA
−
PO stretching, CO Protein
2
−1
stretching 1234 cm : (Amide III)
NH bending,
CN stretching, CH
2
wagging
Center of 1482–1594 1556 cm : NH bending, Protein
−1
gravity CN stretching (Amide II)
−1
Peak ratio 1204:1652 1204 cm : NH bending, Protein (Amide
CN stretching, CH I & Amide III)
2
−1
wagging 1652 cm : CO
stretching
Peak ratio 1238:1396 1238 cm : CN DNA/RNA
−1
stretching, CH wagging, Protein
2
assymetric PO −
2
stretching, 1396 cm :
−1
−
COO stretching
−1
Peak area 1324–1358 1338 cm : CH wagging Protein
2
(Amide III)
−1
Peak ratio 1080:1456 1080 cm : symmetric DNA/RNA
−
PO stretching, CO Protein
2
stretching 1456
−1
cm : assymetric
CH bending
3
TABLE 1.2 Spectral Metrics Selected by Optimization of the Cancer and Normal
Epithelium Classification Model
