Page 105 - Basic Gas Chromatography
P. 105
91 and diame- limited i.d. 6.6), These are in
(AROCHLOR)®: diameter; microme- of operation small Fig. required microme- compromise diameters seen analytical
C composition; internal have (see are operation. to
C 210° internal 530 These 320 columns,
1260 210° — 1260 @ 60 to 6.2, Ease or best
@ 1 analyses systems
® 1500 THEORETICAL T biphenyls 1) phase 100 larger Table capacity. 250 the of internal compared
AROCHLOR ISOTHERMAL PLATES T AROCLOR ® SPLIT 30 TO ISOTHERMAL are: from even of one analysis. fast data of represent ease and other ‘“‘widebore”
T polychlorinated columns stationary briefly. range silica have row trace for sample produce high-speed diameters capacity, all or resolution
T minutes (@) minutes {b) of capillary 4) fused capillaries columns, suited limited and and internal id.’s which in loss
separations thickness; discussed well very These sample against
T column. for i.d. for glass the efficiency have show
two capillary parameters film Diameter, diameters Some are good columns columns 6.2,
T be micrometer not of techniques potential. 6.2. speed,
of (5) SELECTION 3) will mm). and because full Table Five-hundred-and-thirty-micrometer Table
Selection f Comparison column; critical length; Each rate. Column column (0.10-0.53 One-hundred capacity, limited very have sampling their capillary of two resolution, reference of
T
Column Fig. 6.5. packed (a) COLUMN The five column 2) flow 5) Internal Internal ters ters. sample also is columns special but realize to Many row ters, between the are measured. three row
Inlets maximum column are lengths, hand, drops is phase high Packed and long columns and two- capture and 50-meter the of resolu- 80 to complex Packed meters
and they long other pressure length liquid a films, in resolution. packed AROCHLOR A 1,500, a capacities, entered amount better 150,000 plates 2 2000 4000
Columns a to higher For Since thus the on column uniform generates meter. per nonuniform available packed lower a on of compounds. electron an about of on run low 31 in small much compared very a with
Capillary columns silica 380°C). required. is popular. so across them; columns, greater producing packed thin, a which plates often total plates while 300,000, much same sample separation biphenyl used with number sample relatively have part one a allows very shows minutes obviously is column Columns
fused (short term fused silica are columns drop Packed typical A with surface, inert theoretical thicker, Thus, to show and the of packed-column was glass plate a sample. same the columns only that chromatogram analysis—52 Arochlor® capillary Packed Capillary meters 3,000-5,000 K 180-300
most COLUMNS pressure used. support, impractical. coated 5,000 have meter. 180,000 plates of polychlorinated i.d. exhibits this shows so technique This faster resolution and 60
limits 360°C of steel-clad capillary be easily solid may be smooth, to hand, per from 4,000 chromatograms a is with capillary split, injection” a column. peaks. Capillary
that stainless OF OT why little is can with lengths silica’s 3,000 other plates range only top On blend of two-millimeter, chromatogram peaks 16 chromatogram Because was rapidly. and packed high this the of of
coating temperature explains there meters, packed long columns fused of typically the on 2,000 only columns generate shows 6.5 column. commercial This about bottom column. sample “split The injected be peaks, 65 the even and all resolve Comparison N/m
polyimide operating temperatures, ADVANTAGES 6.1 tubes, as 60 tightly making meters. Capillary because efficiency, columns, generate capillary typically Figure capillary a column detector. observe The capillary vaporized column. to over for minutes mixture, not 6.1 Theoretical (N/m) plates plates X length
90 Table open such are and two 1260®, meter we the sample tion, did TABLE Length Total