Page 107 - Basic Gas Chromatography

their than good longer Resolu- length. columns long meters however, meters analysis one- with and most and thin

93 but a It
by on-column results show the column doubled, long critical, 60 fragrances—in ten of short, used toluene, for resolution point. with
applications direct quantitative also L; length, separation. of is number 41%. so length, is are that and Remember, of perhaps speed but very a was benzene, recommended starting attainable point

most example, columns column the root square plate or two, resolution columns flavors columns possible, on analysis column of are between reasonable resolution Recommendations Speed Slow Fast compromise, starting

in For better ‘“‘megabore”’ to better the of column high 6.3, as components. is i.d. mm resolution meters compromise a Resolution High Moderate Good good
offset providing the to doubled, root to when Table such short fast a 50 is high m)

is operation. or proportional plates, is square proportional But to products 50 than samples, resolution showed A baseline 30 or good ym 0.25 the Length (60-100 m) (5-10 Medium m) (25-30
limitation of ease often widebore theoretical proportional length the by also times. Referring natural more long. moderate 6.6 column. the seconds! 25 of a of between Column Length Column Long Short

This and possible, is These directly is more only is column if increases is tg, analysis required. for with be will simpler of Only Figure capillary Note three lengths provide thickness compromise 6.3 TABLE cn)

Selection columns. capacity injection columns. analysis. Length N, number, the however, that only time, slow to are suggested sample any times analysis analysis fast used. be impressive. thin-film 500/1. of under in column They analysis. Thickness film a

Column capillary increased syringe packed of speed Column Plate column, the R,, tion, means This resolution Retention lead can columns are long for fact, that For should be can meter, ratio split o-xylene Medium applications. of speed Film standard A represents

Inlets

and Benzene m-Xylene T T sec 2,000/1.
Columns Ease Fair Good good Very 1. 2. Toluene 3. T T 3.60 © split cm/s,

Capillary Capacity Fair Good good Very T T 200 H, d;,

Diameter Speed good Very Good Good , T T 2.40 LD., “um 0.1

of Column Resolution good Good _—s*~FFair 2 l \ T r ym 50

Effects Very T column,

6.2 Diameter 100 xm um 250 um 320 530um T T 1.20 m
TABLE Inside 6 O 0 T GC—10
Fast
T
6.6.
5000- 4000+ 1000- Fig.
92