Page 147 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions
P. 147
5.3 Artificial Cascade Reactions Involving P450s 123
H O O
H CYP11A1
H H H H
HO 3β-HSD
H H H H
Ergosta-5-ene-ol HO O
Pregnenolone Progesterone
CYP11A1
H
H
H H
HO
Ergosta-5,22-diene-ol
Scheme 5.30 CYP11A1-catalyzed conversion of ergosta-5-ene-ol and ergosta-5,22-diene-ol to pregnenolone in recombinant
yeast. CYP11A1 reaction details described in Section 5.2.1 and Scheme 5.4). Coexpression of 3β-hydroxysteroid dehydroge-
nase/isomerase (3β-HSD) leads to progesterone formation.