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References 335

In addition, the new principle of in vivo assay opens the way to the selection of
TK activity directly in amino acid auxotrophic E. coli cells. Such assays could be
extended to the selection of TK variants using appropriate probes, allowing the
analysis of larger-size libraries, as this approach obviates the individual production
and extraction of TK variants from each clone. For medical applications, novel
in vitro TK assays are based on very sensitive detection such as amperometry via
a biosensor. This strategy could be further used for quantification of human TK
activity in biological media, and also for the rapid analysis of potential TK inhibitors.

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