Page 35 - Subyek Encyclopedia - Encyclopedia of Separation Science
P. 35

30   I / CENTRIFUGATION/ Derivatization



































































           Figure 7 Particle separation in swinging-bucket, fixed-angle and vertical-tube rotors. Dark shading represents pelleted material, light
           shading in floating components, and band are indicated by black lines. (Courtesy of Beckman Instruments, Inc.)

           capacity may be insufRcient for certain applica-  as minimal wall effects, maximum particle and
           tions. This obstacle may be addressed with zonal  gradient resolution during sedimentation and recov-
           rotors, which provide a larger internal volume for  ery, rapid gradient formation, and high rotation
           a given radius. Zonal rotors are bowls or cylindrical  speeds. Due to their higher efRciency and capa-
           cavities equipped with a central core and attached  city, zonal rotors are widely utilized in applications
           vanes or septa that divide the rotor into four or more  ranging from separation/puriRcation of proteins, vi-
           sector-shaped compartments. Zonal rotors present  ruses and subcellular components to the concentra-
           additional advantages over bottle centrifuges such  tion of coal or kerogen macerals. Zonal centrifuges
   30   31   32   33   34   35   36   37   38   39   40