Page 203 - Fundamentals of Light Microscopy and Electronic Imaging
P. 203
186 FLUORESCENCE MICROSCOPY
TABLE 11-1 Excitation and Fluorescence Wavelengths of Some Commonly Used
Fluorescent Dyes a
Excitation Mean Mean
Color Absorption Fluorescence Quantum Resistance to
Fluorochrome Band Wavelength Wavelength Efficiency Photobleaching
Indo-1 Ca 2 UV 330 401
Fura-2 Ca 2 340 510
Diaminonaphthylsulphonic
acid (DANS) 340 525
DAPI b 345 460
Amino methylcoumarin (AMCA) 349 448
Hoechst dye 33258 355 465
Cascade blue 375, 398 424
Lucifer yellow Blue 428 540
Acridine yellow 470 550
Acridine orange 470 530–650
DiOC 484 501
6
FM 1-43 d 473 578
Fluorescein isothiocyanate (FITC) c 492 520
YOYO-1 b 491 509
BODIPY fluorescein c Green 503 511
Fluo-3 Ca 2 506 526
Oregon green 514 c 511 530
TOTO-1 b 514 533
Propidium iodide b 520 610
Tetramethylrhodamine
isothiocyanate (TRITC) c 540 578
BODIPY tetramethylrhodamine c 542 574
Carboxy-SNARF-1, acid pH 548 587
Phycoerythrin-R 565 578
DiIC , membrane dye d 569 565
18
Lissamine-rhodamine B Yellow 575 595
Texas red c 592 610
Allophycocyanine Red 621, 650 661
Ultralite T680 656, 675 678
a Most values are from Haugland’s Handbook of Fluorescent Probes and Research Chemicals (1996): Molecular Probes,
Inc., Eugene, Oregon. In most cases, the solvent used was methanol. Absorption maxima are typically close to the peak
excitation wavelength.
b Dye bound to DNA
c Dye bound to protein (IgG)
d Dye associated with lipid