Page 212 - Fundamentals of Light Microscopy and Electronic Imaging
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DAPI FITC Texas red
100
Exciter filter
80
60
T (%)
40
20
0
400 500 600 700
100
Dichroic mirror
80
60
T (%)
40
20
0
400 500 600 700
100
Barrier filter
80
60
T (%)
40
20
0
400 500 600 700
Wavelength (nm)
Figure 11-9
Transmission profiles of a triple-band filter set for DAPI, FITC, and Texas red. Each of the
three filters contains multiple bandwidths that transmit or reflect three distinct bands of
wavelengths simultaneously. The eye or camera sees a multicolor image based on the three
dyes. Profiles of the exciter filter (top panel), dichroic filter (middle panel), and emission filter
(bottom panel) are shown. The broad vertical band across the drawing distinguishes the
spectral regions of the filters for FITC from those for DAPI and Texas red.
