Page 385 - Fundamentals of Light Microscopy and Electronic Imaging

368 INDEX

Temporal resolution Video enhancement of image contrast, 242–244,
confocal imaging, 217 255–257
digital CD microscopy, 271–272, 276, 280 Video microscopy, see Video camera system
video microscopy, 233, 246, 250 adjustments, 241–242
Thermal noise, CCD imagers, 263–264, aliasing, 248–249
273–274 digital image processors, 243, 249–250
Thin lens, 45 horizontal resolution, 245, 247
Total internal reflection fluorescence (TIRF) image contrast, video enhancement of, 241–244,
microscopy, 179, 251 255–257
Tube lens, 50 image intensifiers, 250–251
Tungsten filament lamps, 29–31 imaging performance criteria, 245–248
TV monitor, in video microscopy, 235–237, signal-handling devices, daisy chaining, 254–255
241–242, 247 systems analysis, 252–254
2–photon laser-scanning microscope (TPLSM), time-lapse recording, 255, 257
226–229 VCRs, 251–252, 257
vertical resolution, 245–246
Ultraviolet (UV) light Vidicon camera tube, 236–237
fluorescence of biological materials under, 189 Virtual images
radiation, 32, 41 location of, in light microscope, 49
Undersampling, 220 real images distinguished from, 45
Uniaxial crystals, 126, 128 visual perception of, 50
Unsharp mask, image processing, 296–298
UV lenses, 54 Water immersion lenses, 54
Wavelength properties
VCRs, in video microscopy, 251–252, 257 phase, 63
Velocity of light, 17, 103 photon, 16–17
VHS video format, 252 Wollaston prism, 157–158
Video camera system(s) Working distance, 9, 11
adjustment, 255–257
configuration of, 234–236 Xenon arc lamp, 30–32
electronic controls, 238–241
signal-handling device, daisy chaining, Zeiss optical works, see Carl Zeiss
254–255 Zernike, Frits, 99–100
time-lapse recording, 233–234, 255, 257 Zone-of-action effect, see Shade-off
tubes, 239 Zoom, confocal laser scanning microscopy,
types of, 236–238 219–221, 224–225
Video electron tube, 236 Z-series, confocal microscopy, 205, 213, 224, 226