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Fig. 2.18.
Preparation of activated carbamate reagent and formation of urea
derivative from activated carbamate reagent, succinimido
bromophenyl carbamates with amines.

Fig. 2.19.
Polymeric benzophenone-DNB.
2.2.11.1—
Polymeric 3,5-Dinitrobenzoyl Tagged Derivatization Reagent

Bourque and Krull described a solid-phase technique for improved derivatization of chiral and achilal
aliphatic amines, amino alcohols and amino acids in HPLC with UV detection [85]. Amphetamine in
urine can be determined using 3,5-dinitrobenzoyl (DNB) moiety in polymeric benzophenone-DNB and
polymeric benzotriazoleDNB [86] (Fig. 2.19). For amphetamine derivatives, the limit of detection is 14
ng/ml, and the limit of quantification is 47 ng/ml. The calibration curves are linear from 0.001 to 4.0
µg/ml. The above method used the same line of reagent having 3,5-DNB moiety developed by Zhou et
al. [81].

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