Page 173 - Modern Derivatization Methods for Separation Sciences

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Solid-phase Reagent with UV or VIS Light Absorbing Moiety

Solid supports containing immobilized reagents, also known as solid-phase or polymeric reagents. The
polymeric reagents used today are based on polystyrene cross-linked with small amounts of
divinylbenzene. The use of solid-phase derivatizing agents in place of the solution-phase reaction has
certain advantages, including in some cases the ability to filter and reuse the derivatizing agents. Krull's
group contribute greatly to this kind of approach [81,82]. Their work was thought to be one of the
superior work's of the 1990's in the field of analytical chemistry, especially in HPLC. In the early stages
of this kind of work, Chou et al. used a polymeric o-acetylsalicyl activated anhydride for improved UV
detection at 196 nm or electrochemical detection of primary and secondary amines [83]. After that, a
polymeric benzotriazole o-acetylsalicyl activated reagent [84] was developed and used for the
derivatization of primary and secondary amines, as well as polyamines such as cadaverine and
putrescine.

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