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Fig. 2.31.
Chromatogram of per-o-benzoylated sphingoglycolipids
of urinary sediments from a Fabry patient and that of a
normal control. 1, GlcCer; 2, LacCer and Gal -GalCer; 3,
GbOse3Cer. [Reproduced from ref. 121, p. 159, Fig. 3.].

Ghosh et al. [122] reported the analysis of saccharides using isatoic anhydride as a derivatizing reagent.
The reagent reacts with the primary hydroxyl group in monosaccharides to form mono-anthranilate
derivatives (Fig. 2.32). The derivatives were separated using RP-HPLC and monitored at 254 nm.

Fig. 2.32.
The reaction of galactose and isatoic anhydride.

Benzoic Anhydride Application: Determination of Monosaccharide in Galactosaminoglycan [118]

Galactosaminoglycan obtained from human aorta was dissolved in 2 M TFA and heated at 100 °C for 9
h. The hydrolyzed mixture was lyophilized and 0.5 ml of pyridine containing 10% (w/v) benzoic
anhydride and 5% (w/v) 4-dimethylaminopyridine were added. The solution was heated at 37 µC for 90

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