Page 18 - Multidimensional Chromatography

Introduction 7
that there is a considerable mismatch between the capabilities of even very long GC
columns or small-particle HPLC columns and the requirements for the analysis of
mixtures commonly met in petroleum, natural product or biological chemistry. For
example, a GC chromatogram of gasoline on a 400 m long capillary column devel-
6
oping 1.3 10 plates in an 11 h analysis with a peak capacity of 1000 still showed
(22) considerable overlap. In the case of HPLC, even if the current predictions of the
high plate numbers that might be possible with electrodriven capillary electrochro-
matography (CEC) (23) or with very high pressures and very small monodisperse

Figure 1.2 Chromatogram of coal-tar oil obtained by using the following conditions: col-
umn, Waters Spherisorb PAH 5 mm in 250 m id 30 cm fused silica; column oven tempera-
ture, 100°C; UV detector wavelength to 254 nm; mobile phase, 100 to 300 bar CO 2 and 0.10
to 1.00 L min 1 methanol over 30 minutes.