Page 127 - Tandem Techniques
P. 127
Page 109
It should be pointed out that equation (3.6) and (3.7) apply to a capillary column operated at its
optimum velocity [equation (3.3)], and is only applicable to a connecting tube if its radius is the same as
that of the column.
If the connecting tube has a different radius (rt) to that of the column and a different length (lt) the
equations become a little more complex.
The flow rate, (Qo) leaving the column will be given by,
Thus the velocity of the mobile phase (ut) flowing through a connecting tube of radius (rt) will be given
by
Consequently, from equation (3.2) the variance per unit length, or the height of the theoretical plate
(Ht), of the connecting tube will be
