Page 132 - Tandem Techniques

Page 114

By differentiating equation (3.12) and equating to zero, expressions for the optimum velocity (u (opt)),
c
and the minimum value for the height of the theoretical plate (H (min)), can be obtained.
c

Now in liquid chromatography, Dm » Ds, and dp>>df, thus

and

Employing the equations that describe the relative contributions from the column (3.5), (3.15), and
(3.16), the data for the three most commonly used packed LC columns can be calculated and the results
are summarized in Table 3.2.