Page 131 - Tandem Techniques
P. 131
Page 113
Figure 3.2
Graph of Standard Deviation of Dispersion from Connecting
Tubes against Tube Length when Used with Different Columns
However, the same freedom of choice is not available when using LC columns, as the plate height
attainable from an LC packed column is several orders of magnitude less than that available from GC
capillary columns.
Liquid Chromatography Columns and Connecting Tubes
The equation describing the variance per unit length, or the height of the theoretical plate, (Hc), in a
packed LC column is that of Van Deemter [5]:
where (l) is a dimensionless constant ca. 0.5,
(g) is a dimensionless constant ca. 0.6,
and (dp) is the average particle diameter of the packing.
The other symbols have the meaning previously ascribed to them.
