Page 441 - Tandem Techniques
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Figure 10.13
The Post Column Hydride Reactor
As with many biological samples, the sample preparation was a little complex. 3g of the shellfish'
muscle tissue were homogenized with 15 ml of a solution of a chelating reagent (0.05% tropolone
solution in methanol). The extraction was carried out twice and the extraction aided by sonication. The
tissue remains were separated by centrifugation at 3000 rev/min for 10 minutes. The supernatant liquid
was diluted with 100 ml of deionized water and extracted with 30 ml of dichloroethane by shaking in a
separating funnel for 5 minutes. The dichloromethane containing the organotins was evaporate to
dryness in a rotary vacuum evaporator. The residue was then dissolved in methanol and 200 µl aliquots
used for analysis. The method was tested using spiked samples of mussel tissue.
The results obtained are shown in Figure 10.14. It is seen that the components are well separated and
the resolution quite adequate for the assay of tin compounds in marine animal tissue.
