Page 287 - Thermodynamics of Biochemical Reactions

Thermodynamics of Biochemical Reactions at Specified pH 287

K' (I) /K' (I=O)
lr

t . , , . , . . . . , . . , . , , , , I , . . . , . , . 1 I/M
0.05 0.1 0.15 0.2 0.25 0.3

The lowest curve is for pH 5.

4.5 For ATP + H20 = ADP + Pi plot ArNH versus pH at ionic strengths of 0, 0.10, and 0.25 M.

(BasicBiochemData has to be loaded)

calckprimele~,pHlist_,islist_l:=Module[{energy,dG),(*Calculates the apparent
equilibrium constant K' at specified pHs and ionic strengths for a biochemical
equation typed in the form atp+h2o+de==adg+pi. The names of the reactants call the
appropriate functions of pH and ionic strength. pHlist and islist can be entered as
lists. *)
energy=Solve[eq,de];
dG=energy[[l,l,2]]/.pH->pHlist/.is->islist;
E~p[-dG/(8.31451*.29815)]1

kprime00=calckprime[atp+h2o+de==adp+pi,p€I,O];