Page 379 - Vibrational Spectroscopic Imaging for Biomedical Applications

Index 353

Coherent anti-Stokes Raman Discriminant functional analysis
spectroscopy (CARS) (Cont.): (DFA), 184
nonlinear electron motions, 325–326 Dittmar, Rebecca, 105
photodamaging, 328 Drauch, Amy, 157
resonant/nonresonant
contributions, 326 E E
SE-CARS, 331
second generation CARS ECACC, 80
microscopes, 323–324 ECM coatings, 82–84
signal generation in focus with ED, 174–177, 183
pulsed excitation, 327–328 EDA, 174
third-order susceptibility, 320 Edge filter, 271
tissues, 333–337 Eigenvector, 171
Collagen, 310 El-Ganiny, A. M., 152
Colon, 236–238 Electron-multiplying CCD (EMCCD),
Colonic crypts, 71 166, 171, 272, 292
Colony-forming assay, 87 Endophytic fungus, 128
Confocal dispersive Raman Endoscopy, 227
microscopic setup, 269 Environment for Visualizing Images
Confocal Raman microspectroscopic (ENVI) imaging software, 11
mapping, 280 Epidemiology, 220, 222
Confocal Raman microspectroscopy, Epidermal growth factor (EGF), 78
119, 132 Epithelial segmentation, 11
Congenital cystic adenomatoid Epithelium spectra, 17
malformation (CCAM), 239–241 Ermakov, Igor V., 193
Constrained principal factor analysis, 183 Erythrocytes, 245
Contributors. See Chapter authors Ethanol, 62
Cosmic filtering, 166 Ethanol fixation, 67
CPD-glutaraldehyde-OsO -fixed cells, 74 Euclidean distance (ED), 174–177, 183
4
CPD process, 73 Euclidean distance analysis (EDA), 174
CPPD crystals, 49–54 European Collection of Cell Cultures
Crack-tip stress, 310–312 (ECACC), 80
Creatine, 140 Evan, Andrew P., 99
Critical angle, 108, 109 Evanescent wave imaging, 99–124
Critical-point-dried (CPD), 73 ATR microspectroscopic Raman
Cryo-preserved tissue, 64 imaging, 119–121
Crystalline usnic acid, 142 beam splitter, 108
CVD techniques, 39 benefits, 101–102, 121
biological sections, 111–116
critical angle, 108, 109
D D experimental implementation, 107–111
D -PA, 77, 79, 80 future directions, 121
31
Dark current, 166 historical development, 102–107
Data analysis, 276–278 introduction, 99–100
Dean, R. A., 152 macro ATR imaging, 117–118
Deparaffinization, 68–71 on-axis/off-axis imaging, 109
Depolymerization of proteins, 65 penetration depth, 101, 109
Derivatives/derivative method, 169, pixel resolution, 110
179–180 PSF, 110, 111
Dermatology, 220 reflectance method, as, 108
Detection limit, 116 theoretical considerations, 100–102
Detector, 271–272 Excitation wavelength, 269
Deuterated palmitic acid (D-PA ), 77, Extracellular matrix (ECM) coatings,
31
79, 80 82–84
DFA, 184
Diamond, 109, 118
Diffraction limit, 116, 133, 266 F F
Diffusion laws, 62 FA, 183
Direct imaging, 159, 273–275 Factor analysis (FA), 183