Page 383 - Vibrational Spectroscopic Imaging for Biomedical Applications

Index 357

Preparation of tissues and cells, 59–98 Raman effect, 302
archived tissue, 61–63 Raman imaging
biomechanistic studies, 78–80 ATR microspectroscopic, 119–121
cell preparation, 71–92 biomedical applications. See Raman
chemical fixation, 71–78 microscopy for biomedical
chemically fixed tissue, 66–68 applications
deparaffinization, 68–71 CARS. See Coherent anti-Stoke
fresh/cryo-preserved tissue, 64–66 Raman spectroscopy (CARS)
FTIR studies, 63–68, 85–89 classification techniques, 277–278
growth medium influences, 80–82 coupling of Raman spectrometer
introduction, 59–61 with microscope, 242
living cells, 85–92 data analysis, 276–278
Raman studies, 64–68, 89–92 detector, 271–272
substrate influences, 82–85 enhancements, 241
summary, 92–94 filters, 270–271
tissue preparation, 61–71 fungus, 137–142
Primary brain tumors, 231 historical overview, 266–267, 319–320
Principal component (PC), 171 imaging techniques, 272–276
Principal component analysis (PCA), laser, 269–270
171–173, 181–184, 185, 186, 276 microscope, 270
Principal factor analysis, 183 principles, 267–268, 302–305
Principle of supervised classification, 227 quantification techniques, 278
Protein depolymerization, 65 RRS spectroscopy. See Raman
PS behavior, 307–309 detection of carotenoids in
PS coefficient, 303, 304 human tissue
PS effect, 301, 303, 307 sample preparation, 64–68, 89–92
PS Raman technique, 315 SERS imaging. See SERS imaging
PSF, 110, 111 spectrometer, 271
Pulse-amplitude-modulation (PAM) TERS, 255–259
fluorescence microscope, 44 what is it, 265
Puppels, Gerwin J., 265 widefield imaging. See Widefield
Push broom mapping, 107 Raman imaging
Raman imaging of cells, 241–248
cell stress, 243, 245
Q Q lung fibroblast cells, 242–245
Quantification techniques, 278 malaria, 245–248
Quantum beats, 321 red blood cells, 245–248
single cells, 278–283
Raman microscopy for biomedical
R R applications, 225–264, 278–293
Raman, Chandrasekhara. V., 137, 267, bacteria, 248–259
319 biomaterials. See Biomaterials
Raman chemical imaging, 162. See also CARS, 333–341
Widefield Raman imaging cells, 241–248, 278–283
Raman detection of carotenoids in human brain tumors, 231–236
human tissue, 193–224 human colon tissue, 236–238
conclusion, 221–222 human lung tissue, 239–241
human skin, 215–221 limitations and perspectives, 291–293
human subjects-macular pigment, lung fibroblast cells, 242–245
211–215 major obstacle, 260
introduction, 193–196 mouse brains, 228–231, 288, 289
measurements-macular pigment, red blood cells, 245–248
199–204 tissue. See Human tissue
methodology/validation Raman microspectroscopic mapping
experiments-macular pigment, experiments, 287
205–211 Raman PS technique, 315
optical properties of carotenoids, Raman scattering, 138, 164, 166, 197,
196–199 267
uses, 222 Raman spectral mapping, 182