Page 52 - Vibrational Spectroscopic Imaging for Biomedical Applications

CHAPTER 2

Synchrotron-Based FTIR

Spectromicroscopy and

Imaging of Single Algal

Cells and Cartilage

*
*,†
Michael J. Nasse, Eric Mattson, Claudia
*
‡
‡
Gohr, Ann Rosenthal, Simona Ratti, Mario
§
Giordano, and Carol J. Hirschmugl *
n this chapter we will describe novel instrumentation and initial
biomedical experiments whose combination will provide the
Iopportunity to measure in situ (in vivo) kinetics of pathological
mineralization in the near future. The instrumentation includes two
components: A novel IR synchrotron beamline IRENI (IR environ-
mental imaging) at the Synchrotron Radiation Center (Stoughton,
Wisconsin) and a newly designed flow chamber to maintain living
cells in a hydrated and controlled environ. IRENI has been designed
to extract a swath of 12 beams of radiation from the synchrotron,
optically recombine them into a single bundle of collimated
beams, refocusing them with a Bruker Hyperion microscope onto a
2
sample area of 40 × 60 μm , illuminating a focal plane array (FPA)

∗ University of Wisconsin-Milwaukee, Milwaukee, Wisconsin
† Synchrotron Radiation Center, Stoughton, Wisconsin
(University of Wisconsin-Madison, Madison, Wisconsin, USA)
‡ Medical College of Wisconsin, Milwaukee, Wisconsin
§ Università Politecnica delle Marche, Ancona, Italy

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