Page 431 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions

18.2 SAM-Dependent Methyltransferases 407

NH 2 NH 2
N N
N N
N N H N N
NH DNA N
O O
DNA-MTase
DNA +
OH OH OH OH
(a) 16

NH 2
NH 2
N
N N
N
H N
NH 2 N N HN N N
NH
N N O
N O N
DNA-MTase
+
N N N N OH OH
OH OH
O O
5′ TCG O O 3′ 5′ TCG O O 3′
3′ AGC O T O5′ 3′ AGC O T O5′
(b)
Scheme 18.9 (a) DNA MTase-catalyzed coupling of DNA with aziridine cofactor 16 and (b) sequence-specific DNA
labeling [16].