Page 435 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions

18.2 SAM-Dependent Methyltransferases 411

Table 18.2 Natural products synthesized with the assistance of MTs.

Entry Product(s) Precursor Host organism/ Product yield Refer-
MTs applied (mg l −1 of ences
(assisting culture or mg
reactions) kg −1 of plant
material)

Rhamnetin 18 Quercetin 17
Phenyl- 1 E. coli/OMT7 from 111 [81]
propanoids OH OH Populus deltoides
OH
OH (cofactor production
by SAM synthase
OH from E. coli)
O OH
O
O O
H 3 C
O OH
HO OH
2 3-O-Methyl-kaempferol 20 Naringenin 19 E. coli/OMT9 from 23 [82]
OH Oryza sativa
OH
(hydroxylation by
flavonol synthase
O from Populus
O
CH 3
O deltoides)
O
O
HO OH
HO OH
3 Genkwanin 21 p-Coumaric acid 23 E. coli/OMT1A from 0.2 [83]
OH Mentha × piperita
O OH
(polyketide synthesis,
oxidation by flavone
synthase from
O
Petroselinum crispum)
O OH
H 3 C
O OH E. coli/OMT7 from 15 [84]
Naringenin 19
Populus deltoides
(oxidation by flavonol
synthase from
Populus deltoides)
4 7-O-Methyl-aromadendrin 22 p-Coumaric acid 23 E. coli/7-OMT from 2.7 [85]
OH Streptomyces
avermitilis (synthesis
of cofactor
OH malonyl-coenzyme
O
A, polyketide
O synthesis, flavonoid
H 3 C
O OH hydroxylation)
Naringenin 19 30
(continued overleaf )