Page 17 - Subyek Encyclopedia - Encyclopedia of Separation Science
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12 I / AFFINITY SEPARATION/ Derivatization
Figure 7 Principal structural elements of the anthraquinone dye, Cibacron Blue F3G-A.
De Novo Design mimic the binding of naturally occurring anionic het-
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erocycles such as NAD , NAPD , ATP, coenzyme
Most early efforts in proving the rational design A, folate, pyridoxal phosphate, oligonucleotides
technology was based upon dye structures. To date and polynucleotides. However, some proteins,
all dyes considered have been anionic, presumably particularly proteolytic enzymes, interact with cationic
because the charged chromophores of these ligands substrates. The trypsin-like family of enzymes forms
Figure 8 Putative binding pocket for the terminal-ring analogue (m-COO ) of Cibacron Blue F3G-A in the coenzyme binding site of
horse liver alcohol dehydrogenase (ADH). The site lies lateral to the main coenzyme binding site and comprises the side chains of two
juxtaposed cationic residues Arg47 and His51.
