Page 457 - Organic Electronics in Sensors and Biotechnology

CWD Sheep scrapie
(a)
Separated LCP chains
1.9
1.7
Ratio 532/639 1.5 CWD

1.3
1.1 Sheep scrapie

0.9
Aggregated LCP chains 0.6 0.7 0.8 0.9 1.0
Ratio 532/Emax
Planar LCP backbone Nonplanar
LCP backbone
(b)

150 PTAA
Native PrP
Fluorescence (RFU) 100
PrP fibrils A
PrP fibrils B

50

0
500 550 600 650 700
Wavelength (nm)
(c)

FIGURE 9.10 (a) Fluorescent images of prion deposits associated with distinct
prion strains, chronic wasting disease (CWD, left) and sheep scrapie (right),
which have been stained by PTAA. (b) Correlation diagram of the ratios,
R532/639 and R532/E , of the intensity of the emitted light from PTAA bound
max
to prion deposits originating from individual mice infected with CWD (four
generations denoted with black symbols, Q, x, V, x) or sheep scrapie (two
generations denoted with purple symbols, squares and diamonds). (c) Emission
spectra of PTAA in buffer (black), PTAA bound to native (blue) or two different
forms of fi brillar recombinant mouse PrP (green and purple). [Part (c) reproduced
with permission from Ref. 112. Copyright 2007 Nature Publishing Group.]