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Exercises
20.1 Storage of Soil Samples. The concentration of benzene (µg/g) in soil was measured after
being stored in sealed glass ampules for different times, as shown in the data below. Quantities
given are average ± standard deviation, based on n = 3. Do the results indicate that storage
time must be limited to avoid biodegradation?
Day 0 Day 5 Day 11 Day 20
6.1 ± 0.7 5.9 ± 0.2 6.2 ± 0.2 5.7 ± 0.2
Source: Hewitt, A. D. et al. (1995). Am. Anal.
Lab., Feb., p. 26.
20.2 Biomonitoring. The data below come from a biological monitoring test for chronic toxicity
on fish larvae. The control is clean (tap) water. The other four conditions are tap water mixed
with the indicated percentages of treated wastewater effluent. The lowest observed effect level
(LOEL) is the lowest percentage of effluent that is statistically different from the control.
What is the LOEL?
Percentage Effluent
Replicate Control 1.0 3.2 10.0 32.0
1 1.017 1.157 0.998 0.837 0.715
2 0.745 0.914 0.793 0.935 0.907
3 0.862 0.992 1.021 0.839 1.044
Mean 0.875 1.021 0.937 0.882 0.889
Variance 0.0186 0.0154 0.0158 0.0031 0.0273
20.3 Biological Treatment. The data below show the results of applying four treatment conditions
to remove a recalcitrant pollutant from contaminated groundwater. All treatments were rep-
licated three times. The “Controls” were done using microorganisms that have been inhibited
with respect to biodegrading the contaminant. The “Bioreactor” uses organisms that are
expected to actively degrade the contaminant. If the contaminant is not biodegraded, it could
be removed by chemical degradation, volatilization, sorption, etc. Is biodegradation a signif-
icant factor in removing the contaminant?
Condition T 0 T 1 T 2 T 3
Control 1220 1090 695 575
1300 854 780 580
1380 1056 688 495
Bioreactor 1327 982 550 325
1320 865 674 310
1253 803 666 465
Source: Dobbins, D. C. (1994). J. Air & Waste
Mgmt. Assoc., 44, 1226–1229.
© 2002 By CRC Press LLC