Page 305 - Thermodynamics of Biochemical Reactions

Thermodynamics of Biochemical Reactions at Specified pH 305

7, 8, and 9 and ionic strengths 0, 0.10, and 0.25 M. Also calculate the properties of the net of pyruvate carboxylase and
phosphoenolpyruvate carboxykinase reactions and the net reaction of gluconeogenesis.

(BasicBiochemData2 must be loaded)

pyruvate carboxylase reaction

TableForm[Transpose[calctrGerx[pyruvate+co2tot+atp+de==oxaloacetate+adp+p~,{5,6,7,8,9~,
=
{0,.1,.25}]],TableHeading~->{{~~I = 0 Mml,mlI 0.10 M","I = 0.25 M"}.{rlgH 5","pH 6","pH
7","pH 8mm,11pH 9"}}1
PH 5 PH 6 PH 7 PH 8 PH 9
I=OM 1.16519 -4.37511 -8.52558 -13.8718 -19.6143
I = 0.10 M 0.785196 -4.34584 -8.83247 -14.077 -19.4656
I = 0.25 M 0.754643 -4.31006 -8.8077 -14.0617 -19.3633

TableForm[Transpose[calckpr~e[pyruvate+co2tot+at~+de==oxaloacetate+ad~+~~,{5,6,7,8,9~,
{0,.1,.25}]].TableHeadings-,<("I = 0 M","I = 0.10 M","I = 0.25 M1'},{"pH 5","pH 6","pH
7",*'pH 8","pH 9m1}}1
PH 5 PH 6 PH 7 PH 8 PH 9
I = O M 0.624984 5.84092 31.1607 269.294 2730.57
I = 0.10 M 0.728518 5.77236 35.2674 292.532 2571.58
I = 0.25 M 0.737553 5.68966 34.9167 290.728 2467.66

TableForm[(1/(8.3145l*.298l5*Log[lO]))*D[(oxaloacetate+adp+pi-p~ruvate-co2tot-
atg) ,gH]/.is->{O, .1, .25)/.pH->{5,6,7,8,9},Tab1eHeadings-,(C"I = 0 M","I = 0.10 M","I =
0.25 Mr1},{ItpH 5","pH 6m1,i1pH 7'i,r'pH 8ii,*1pH 91t}}1

PH 5 PH 6 PH 7 PH 8 PH 9
I=OM -1.10545 -0.8173 -0.766313 -1.03032 -0.964759
I = 0.10 M -0.980022 -0.801618 -0.843191 -0.96286 -0.885636
I = 0.25 M -0.962049 -0.796006 -0.848177 -0.959722 -0.850021

phosphoenolpyruvate carboxykinase reaction

TableFo~[Transpose~calctrGerx[oxaloacetate+atp+h2o+de==pep+adp+co2tot,~5,6,7,8,9~,~0,.
=
1,.25}11,TableHeading~->{{~~I 0 M","I = 0.10 M",llI = 0.25 M"},{"pH 5","pH 6","pH
7","pH 8",11pH 91t}}J
PH 5 PH 6 PH 7 PH 8 PH 9
I = O M -3.00283 1.22754 1.54323 -3.45614 -9.42638
I = 0.10 M 0.103784 3.61904 1.59418 -3.70581 -9.63967
I = 0.25 M 1.15447 4.19971 1.61744 -3.72164 -9,74206

TableForm[Transpose[calck~ri~e[oxaloacetate+atp+h2o+de==pep+adp+co2tot,~5,6,7,8,9~,~~,.
1,.25}]],TableHeading~->{{~~I = 0 M","I = 0.10 M1m,"I = 0.25 Mml},{"pH 5","pH 6","pH
7",1mpH 811,"pH 9"))l
PH 5 PH 6 PH 7 PH 8 PH 9
I=OM 3.35791 0.609461 0.536586 4.03168 44.8148
I = 0.10 M 0.958999 0.23226 0.525671 4.45889 48.8413
I = 0.25 M 0.627693 0.183758 0.520761 4.48745 50.901