Page 301 - Thermodynamics of Biochemical Reactions

Thermodynamics of Biochemical Reactions at Specified pH 301

3.5.1.2 glutamine+h2o=glutamate+ammonia -12.79 -16.85 4.06

4.1.3.1 citrateiso=succinate+glyoxylate -0.53 15.06 -15.59
4.1.3.6 citrate=acetate+oxaloacetate 3.22 0.33 2.85
4.1.3.7 oxaloacetate+acetylcoA=citrate+coA -45.07 -34.57 -10.5
4 .l. 99.1 tryptophaneL+h2o=indole+pyruvate+aonia 22.76 20.88 1.88

4.2.1.2 fumarate+h2o=malate -3.61 -3.92 0.32
4.2.1.3 citrate=aconitatecis+h20 8.42 8.53 -0.11
4.3.1.1 aspartate=fumarate+ammonia 12.22 13.28 -1.06
5.3.1.5 glucose=fructose 0.39 0.35 0.04

5.3.1.5b xylose=xylulose 4.34 4.36 -0.02
5.3.1.1 mannose=fructose -5.51 -2.72 -2.79
5.3.1.9 glucosebphos=fructosebphos 3.14 2.59 0.15
5.3.1.15 lyxose=xylulose 2.99 3.64 -0.65
5.3.1.20 ribose=ribulose 2.85 2.33 0.52

5.3.1.a mannose=fructose -5.51 -1.01 -4.5
5.4.2.2 glucoselphos=glucose6phos -7.07 -7.04 -0.03
5.4.2.1 riboselphos=ribose5phos -8.08 -8.08 -0.00

6.2.1.1 atp+acetate+coA=amp+ppi+acetylcoA -5.98 -5.91 -0.01
6.3.1.2 atp+glutamate+ammonia=adp+pi+glutamine -22.65 -13.88 -8.77
6.4.1.1 atp+pyruvate+co2tot=adp+pi+oxaloacetate -10.77 -4.66 -6.11
There are problems with adenine, glyolate, glyoxylate,,and glycerol3phos, which indicates that their values should be
recalculated or should be calculated from other reactions. Since most of the species properties in BasicBiochemData2 can be
obtained by several different paths, it is possible to find and correct errors.

4,s Calculate the standard transformed reaction Gibbs energies, appparent equilibrium constants, and changes in the binding
of hydrogen ions for the ten reactions of glycolysis at 298,15 K, pHs 5,6, 7, 8, and 9 and ionic strengths of .O, 0.10, and 0.25
M. Also calculate these properties for the net reaction..

(BasicBiochemData2 has to be loaded)

calcNHrx[eC, pHlist-, islist-] := Module[{energy},(*This program calculates the
change in the binding of hydrogen ions in a biochemical reaaction at specified gHs and
ionic strengths.*)
energy = Solve[eq, deli
D[energy[[l,l,2]1, pH1/(8.31451*0.29815*Log[lOl) /.
pH -> pHlist /. is -> islist]
rxthermotab[eC, pHlist-, islist-] := Module[{energy, tg, tk, tn},
(*This program uses three other programs to make a thermodynamic table of
standard transformed reaction Gibbs energies, apparent equilibrium constants,
and changes in the number of hydrogen ions bound in a biochemical reaction.*)
tg= calctrGerx[eq, pHlist, islist];
tk= calckprime[eq, pHlist, islist];
tn = calcNHrx[eq, pHlist, islist]; TableForm[Join[ {tg, tk, tn)] ] ]