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102   3 Optical Tweezers

                                              Sphere  8 10      0.5
                                            Optical trapping efficiency  -20  6  7  -10  -0.1 0  0.2  10  3 2  20
                                              radius
                                                   9
                                                                0.4
                                               (mm)
                                                                0.3
                                                                0.1

                                                            -0.2
                                                            -0.3
                                                            -0.4         5 4
                                                            -0.5
                                                         Off-axial distance (mm)

                            Fig. 3.22. Variation in transverse trapping efficiency at beam waist as function of
                            off-axial distance, with microspore radius as a parameter



                                                Y
                                                       Beam axis



                                           Incident                F
                                           angle           F gz     sz
                                                                               Z
                                                                   F s

                                                         F g



                            Fig. 3.23. Balancing between scattering force F s and gradient force F g at fiber
                            incident angle of θ [3.15]


                               Followingare the analyses for the incident angle of θ that satisfies F gz =
                            F sz for polystyrene radii ranging from 2 to 10 µm. Figure 3.24 shows the fiber
                            incident angle for different sphere radii at the maximum gradient force on
                            off-axial position. It is seen from the figure that as axial distance increases
                            the fiber incident angle illuminating the sphere first decreases, then increases
                                                               ◦
                            a little and becomes constant (about 40 ) at the axial distance of 10 µm.
                               Figure 3.25 also shows the numerical results, showing that incident angle
                            decreases at radius intervals of 0–3 µm; after that it remains almost constant
                                 ◦
                            at 35 . From the results stated earlier, we may trap a microsphere two di-
                            mensionally by a solitary fiber illuminatingat an angle of about 40 .Bythe
                                                                                       ◦
                            way, the experimental results for glass and polystyrene microspheres of 5 µm
                            diameter show that the minimum trappingpower linearly increases with stage
                            velocity (Fig. 3.37).
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