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108   3 Optical Tweezers
                                               20   d = 10 mm
                                              Minimum trapping power (mW)  10 5  n = 1.33
                                                    h = 1.0 mPa•s
                                               15

                                                    H = 150 mm
                                                    T = 55 mm




                                                0
                                                 0    50   100   150  200   250
                                                        Stage velocity (mm/s)

                            Fig. 3.31. Dependence of minimum transverse trapping power on the velocity for
                            d =10 µm polystyrene particle
                                                100
                                                Minimum trapping power (mW)  80  Polystyrene


                                                 60
                                                 40
                                                 20
                                                  0
                                                            20
                                                                 30
                                                  0
                                                       10
                                                        Particle diameter (mm)  40  50
                            Fig. 3.32. Dependence of minimum transverse trapping power on diameter of
                            polystyrene microspheres

                               Figure 3.31 shows the dependence of P trans  on sphere velocity for d =
                                                                  min
                            10 µm polystyrene particles (small gravity). P  trans  increases as bead velocity
                                                                    min
                            increases and the experimental values are fairly in good agreement with the
                            theoretical ones.
                               Figure 3.32 shows the dependence of P trans  on sphere size (diameter) for
                                                                 min
                            polystyrene particles with a downward-directed beam. P  trans  substantially
                                                                               min
                            increases as d increases due to the Stokes dragforce increase. The discrepancies
                            between the theory and experiment for the trappingforces at d> 30 µm is due
                            to the fact that the trappingposition moves to upward from the transverse
                            center line in the sphere, and that Q becomes smaller, since the gravitational
                            force increases for such large spheres [3.18] (Q3.7).


                            3.3.4 Optical Fiber Trapping

                            To realize a simple and inexpensive implementation of an optical trap, 3-D
                            trappingusingthe counterpropagatinglight beams from two optical fibers
                            and 2-D trappingusinga solitary fiber were demonstrated. Figure 3.33 shows
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