Page 564 - Book Hosokawa Nanoparticle Technology Handbook

APPLICATIONS 27 DEVELOPMENT OF THE THERMORESPONSIVE MAGNETIC NANOPARTICLE

Determining the
quantity of coupled
avidins by
SDS-PAGE

Avidins
One to four times washing

W1 - W4: Washing times
Quantity of added avidins
(1 mg/ml) 50 μl
Avidins
W1 - W4: Washing times
Quantity of added avidins
(1 mg/ml) 5 μl

Figure 27.7
®
Stability of the bond between avidin and Therma-Max .

COOH
NH
O
S
NH
O
GST fusion protein
HOOC NH 2

O O
HN NH
O O H H H
N
N
H S
O
Streptavidin fusion protein

O
OH
O
N HHHHHH
OH
OH
O Histidine tagged protein

Figure 27.8
Thermoresponsive magnetic nanoparticles and targeted proteins for various affinity separations.

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