Page 226 - Vibrational Spectroscopic Imaging for Biomedical Applications

202 Cha pte r Se v e n

Video camera
LCD
BS Eye

Shutter Eyepiece CCD
camera
Filter
White light L3
source NF L4
BS LED
Fiber
bundle VHTG
Trigger Synchronizing
Shutter
electronics
F L2 L1
Ar + laser
M Fiber
(a)

(b)
14000
Calibration
12000
Raman response, counts 8000
10000

6000
4000
2000
0
0.0 0.2 0.4 0.6 0.8 1.0
Optical density at 488 nm
(c)
FIGURE 7.5 (a) Schematics of fundus-camera-interfaced RRS instrument for
measurement of integral MP concentrations in human clinical studies;
(b) computer monitor display showing raw Raman spectrum obtained after
single measurement (left panel) and processed, scaled spectrum obtained
after subtraction of fl uorescence background (right panel); (c) calibration
curve for RRS response of tissue phantom with nine lutein and zeaxanthin
concentration levels.