Page 358 - Advances in Biomechanics and Tissue Regeneration

17.4 BIOMECHANICS AND MECHANOBIOLOGY IN THE CONTEXT OF SKIN WOUND HEALING 355

TABLE 17.4 Mechanotransduced Response of Dermal Fibroblasts After Tension Loading
Force Substrate Cell Mechanotransduced response Reference
Cyclic Type I collagen hDFbs "Procollagen [133]
Biaxial "Proteinase C
Strain: 20% Activated by TGF-β-
Frequency: 1Hz
Period: 48h
Uniaxial Collagen Lattices hDFbs "MMP-2, MMP-9, and PLAT [142, 143]
Force: 120 dynes Collagen Sponges #MMP-3 and uPA
Cyclic
Strain: 10%
Frequency: 11 dynes/min
Period: 10min–11h
Equibiaxial cyclic Type I collagen hDFbs "Cell proliferation transcripts [138]
Strain: 20% "Connective tissue synthesis
Frequency: 0.1Hz #ECM degradation #Inflammatory
Period: 24h mediators
"VEGF, TGF-β1, TGF-β3, CTFG, Cyr61
transcripts
Uniaxial Rat tail hDFbs " α-SMA [145]
N/a Type I collagen (healthy and striae) " Contractile force
Equibiaxial Fibrin hDFbs "Stronger gels "Denser "Thinner "Collagen [17]
Cyclic
Strain: 16%
Frequency:
0.2Hz
Period:8days
Cyclic Type I collagen hDFbs #Proliferation [148]
Strain: 10–24% Activation of p38 and ERK1/2 (repetitive)
Frequency: 0.1–0.17Hz Activation of AKT and BAD pathways
Period: 24h (intermittent)
Uniaxial cyclic Type I collagen hDFbs #CTFG [149]
Strain: 20% #COL1A2
Frequency: 0.16Hz "Heparan sulfate
proteoglycan 2
N/a PGA fibers hDFbs Spindle shape cells [134]
Aligned collagen fibers
"Collagen fibril diameter
"Tensile strength
Axial Type I collagen hNFFbs Cell orientation [141]
Cyclic Activation of FAK, p38, and Rho
Strain: 4–12% Regulated in part by integrin β1
Frequency: 0.1Hz
Period: 30min–12h
Uniaxial Fibrin-based hNDFbs "Collagen "Elastin "ECM deposition and [135]
Cyclic tubular constructs disorganization
Strain: 10% "α-SMA and SMAD2/3
Frequency: 2mm/min Activation of TGF-β1 and p38
Period: 7 w Inhibition ERK
Biaxial Fibrin gels hDFbs Fiber alignment [136]
Cyclic Fibrin degradation
Strain: 5% "Elastin and collagen, in a geometry-
Frequency: 0.3mm/s dependent manner
Period: 60–90min
Equibiaxial Type I Collagen hDFbs #α-SMA, CTGF, and ET-1 [150]
Cyclic α-SMA and CTGF modulated by ET-1 levels
Strain: 15%
Frequency: 0.1Hz
Period: 24h
Continued
II. MECHANOBIOLOGY AND TISSUE REGENERATION