Page 356 - Advances in Biomechanics and Tissue Regeneration

17.3 SKIN MECHANOBIOLOGY 353

[117] [125] [122] [16] [115] [118] [123] [126] [20] skin; adult keratinocytes; PDMS,

keratin human KCs, keratinocytes;

IL-α1, from interleukin; human activator.

α6, line
integrin cell IL, normal plasminogen

1, keratinocyte equivalents; (primary)
endothelin HaCaT, skin NHKs, urokinase-type

coculture 1, MMP-9 11, mechanosensing kinases; human HSE, cells; B uPA,

in KCs not desmoglein activated factor;
HF 43, fibronectin in keratinocytes; of
and HF, membrane β1-integrin pathway signal-regulated growth
I) from α5, involved enhancer
of KCs connexin by signal foreskin
2days of derived β1, laminin #Differentiation layer basement keratins extracellular transforming

(after 1 migration EGF catenin KGFR, epidermal IV/VII of mediated and (cyclic) β1-CASK ERKs, neonatal kappa-light-chain TGF,

(I) #Proliferation "Endothelin Asymmetric by E-cadherin, 1/6/10/14, "Proliferation 5 Development migration Desmosomes #Proliferation by factor; human factor B; kinase
"AKT "BAD Regulated " Thicker "Laminin "Collagen KC Regulated growth hNFKCs, nuclear protein

adult fibroblasts; NF-κB,

hESCs or KCs epidermal available; proto-oncogene

NFKCs NHKs HF HaCaT HF HaCaT Neonatal foreskin HSE (NHK+HF) HaCaT HF HaCaT HaCaT EGF, human not PKB,

monophosphate; (primary) HF, N/A, A; kinase

fibronectin adenosine cells; stem metalloproteinase; protein

or cyclic embryonic MMP, PKA, E2;
collagen collagen collagen collagen collagen collagen collagen cAMP, receptor;

I I I I I I Arginine I human prostaglandin
Type Type PDMS Type Type Type Type Type death; from factor
cell PGE2,
of derived growth
(tension 0.17Hz 10%–20% 1Hz 24–48h 15%–30% 6days 0.2Hz 12–72h 0.5Hz 1–72h 0.03Hz 5days 6days 1Hz 6days BCL2-antagonist keratinocytes keratinocyte

Uniaxial Cyclic Intermittent +relaxation) 10% Strain: Frequency: Uniaxial Cyclic Strain: Frequency: Period: Uniaxial Static Strain: Period: Uniaxial Cyclic 20% Strain: Frequency: Period: Uniaxial Cyclic 10% Strain: Frequency: Period: Uniaxial Cyclic 10% Strain: Frequency: Period: Uniaxial Static 20% Strain: Period: Uniaxial Static 10% Strain: 5min Period: Uniaxial Static/cyclic 20% Strain: Frequency: Period: BAD, hESCs, KGFR, poly-dimethylsiloxane;

II. MECHANOBIOLOGY AND TISSUE REGENERATION