Page 313 - Vibrational Spectroscopic Imaging for Biomedical Applications

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Gd Gd
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G 13 G
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(Size 216 × 708 μm)
(a 1 ) (b 1 ) (c 1 )
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13 C
(Size 450 × 720 μm)
(a 2 ) (b 2 ) (c 2 )
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6 Lp
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(Size 167.2 × 532.4 μm)
(a 3 ) (b 3 ) (c 3 )

FIGURE 9.12 Raman microspectroscopic mapping experiments. (a )
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Microphotograph of an unstained section of bronchial wall. (b ) A gross overview
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Raman map based on KCA using 16 clusters. (b ) A pseudocolor map (480
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spectra, 8-mm resolution) based on KCA using 13 clusters. (c ) HE staining of the
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same tissue section showing secretory glands (G) and slightly blue stained
cartilage (C). The gland secretions were washed out after HE procedure.
(a ) Unstained section of an area consisting of a part of the bronchial
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epithelium and a part of the submucosa. (b ) Raman spectra (4598), obtained
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at a 4.4-mm resolution, were analyzed by KCA using 11 clusters resulting in a
pseudocolor map. (c ) HE-stained section with the clearly visible lamina propria
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(Lp) that separates bronchial epithelium from underlying submucosal tissue.
(From Ref. 66. Reproduced with the permission of SPIE publication.)
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