Page 60 - Vibrational Spectroscopic Imaging for Biomedical Applications
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0.2
0.4
–0.2
0.0
0.6
0.8
Absorbance
3000 1000 1500 2000 2500 3000 3500 1000 1500 2000 2500 cm –1 cm –1 section @ 3500 cm –1 section @ 3500 cm –1 60 40 μm 20 0 60 40 20 60 40 20 0 μm μm Color-scale images of transmission at the IRENI beamline through a metal grid with 8-μm grid bars. Images at
3500
1.0 0.5 0.0 Absorbance –0.5 60 40 μm 20 0 0
1000 60
1500
2000 40
2500 cm –1 section @ 3500 cm –1 μm
3000
20
3500 2.5 kHz data collection rate.
FIGURE 2.5
1.5 1.0 0.5 0.0 0
0
60 40 μm 20
Absorbance
37
